Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

In vitro assessment of activity of graphene silver composite sheets against multidrug-resistant bacteria and Tomato Bushy Stunt Virus

Ahmed M Elazzazy^1,2 , Essam KF Elbeshehy^1,3, Mohamed A Betiha⁴

¹Biological Sciences Department, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia; ²Chemistry of Natural and Microbial Products Department, National Research Center, Dokki, Giza; ³Department of Agricultural Botany, Faculty of Agriculture, Suez Canal University; ⁴Egyptian Petroleum Research Institute, Nasr City, Cairo 11727, Egypt.

For correspondence:- Ahmed Elazzazy Email: Ahmedazazy8@hotmail.com Tel:+966560922828

Accepted: 24 October 2017 Published: 30 November 2017

Citation: Elazzazy AM, Elbeshehy EK, Betiha MA. In vitro assessment of activity of graphene silver composite sheets against multidrug-resistant bacteria and Tomato Bushy Stunt Virus. Trop J Pharm Res 2017; 16(11):2705-2711 doi: 10.4314/tjpr.v16i11.19

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To synthesize graphene-based silver nanocomposites and evaluate their antimicrobial and anti-Tomato Bushy Stunt Virus (TBSV) activities.

Methods: A graphene-based silver composite was prepared by adsorbing silver nanoparticles AgNPs to the surfaces of graphene oxide (GO) sheets. Scanning electron microscopy was used to analyze the morphology of the synthesized graphene-based silver nanocomposite. This compound was investigated for its antimicrobial activity against several multidrug-resistant human pathogens using the agar well diffusion technique. Moreover, the biocompatibility and antiviral activity of the graphene-based nanocomposite against TBSV was studied in lettuce.

Results: The graphene-based silver composite exhibited remarkable antimicrobial effects against pathogenic bacteria including Shigella sonnei and Pseudomonas aeruginosa with zones of inhibition of 32 ± 0.11 and 29 ± 0.05 mm, respectively. They inhibited TBSV better than graphene and GO.

Conclusion: The synthesized graphene-based silver composite exhibits potent activity against TBSV and multidrug resistant bacteria, indicating that they are good candidates for future therapeutic applications

Keywords: Graphene oxide, Graphene-based nanocomposite, Antiviral, Antimicrobial, Multidrug-resistant (MDR) human pathogens